Table 5.
Purity ratio of phycocyanin after different purification steps.
| Purification step | Purity ratio (A620/A280) |
Phycocyanin conc. (mg/mL) | Phycocyanin recovery (%) |
|---|---|---|---|
| Crude phycocyanin extract | 0.60 ± 0.14 | 0.83 ± 0.19 | |
| Microfiltration | 0.63 ± 0.09 | 0.66 ± 0.05 | |
| Ultrafiltration | 1.22 ± 0.18 | 6.43 ± 0.53 | 100 |
| One-step chromatography column: | |||
| Activated charcoal | 1.34 ± 0.09 | 5.72 ± 0.23 | 88.9 |
| Sephadex G100 | 2.77 ± 0.38 | 3.34 ± 0.37 | 51.9 |
| DEAE Sepharose Fast Flow | 3.25 ± 0.29 | 3.51 ± 0.36 | 48.2 |
| Two-step chromatography columns: | |||
| Sephadex G100 and DEAE Sepharose | 3.74 ± 0.18 | 1.40 ± 0.02 | 21.8 |
| DEAE Sepharose and Sephadex G100 | 3.82 ± 0.16 | 1.63 ± 0.04 | 25.3 |