FIG. 4.

K562 cyclin D1-CAT stable transfectant cell cycle synchronization and transcription analysis. (A) Flow cytometry analysis of DNA contents of counterflow centrifugal elutriated cells (FI-FI5) as determined by FACScan. Percentage of G₁ S-phase, and G₂ × M cells in each synchronized cell fraction is shown in each inset. (B) Hybridization of nascent nuclear transcripts to cyclin D1, CAT, and GAPDH DNA probes. (C) Quantitation of nascent cyclin D1 and CAT transcripts normalized with nascent GAPDH transcripts, respectively.