TABLE 2.

SEGREGATION OF hPPARγ GENE IN SOMATIC CELL HYBRIDS, SHOWING hPPARγ SEGREGATES WITH HUMAN CHROMOSOME 3

Human Chromosome	hPPARγ Gene/Chromosome				Discordancy (%)
	+/+	+/−	−/+	−/−
1	19	8	13	45	25
2	17	10	9	49	22
3	27	0	0	58	0
4	26	1	24	34	29
5	18	9	5	53	16
6	21	6	25	33	36
7	11	16	26	32	49
8	18	9	15	43	29
9	20	7	13	45	24
10	11	16	5	53	25
11	17	10	11	47	25
12	12	15	14	44	34
13	14	13	20	38	39
14	13	14	29	29	51
15	17	10	30	28	47
16	11	16	25	33	48
17	21	6	32	26	45
18	21	6	22	36	33
19	20	7	8	50	18
20	21	6	17	41	27
21	25	2	30	28	38
22	16	11	11	47	26
X	18	9	24	34	39

+/+ = number of somatic cell hybrids in which the hPPARγ gene was detected, and human chromosome z was present; +/− = hPPARγ gene detected, chromosome z not present; −/+ = hPPAR gene not detected, but chromosome z present; −/− neither hPPARγ gene nor chromosome z present. The human PPARG gene was detected as 1.35-, 3.4-, and 16.5-kb bands in EcoR I digests of human DNA and human-rodent somatic hybrid cell DNAs after Southern hybridization with a 0.8-kb cDNA probe. The human bands were all well resolved from cross-hybridizing 2.85-, 4.3-, and 15.3-kb, or 2.8- and 10.9-kb bands in EcoR I-digested Chinese hamster and mouse DNAs, respectively. The three human bands were either all present or all absent in any hybrid cell line. Detection of the human gene is correlated with the presence or absence of each human chromosome in the group of somatic cell hybrids. Discordancy represents presence of the gene in the absence of the chromosome (+/−), or absence of the gene despite the presence of the chromosome (−/+), and the sum of these numbers divided by the total number of hybrids examined (×100) represents percent discordancy. The 41 human–hamster hybrids consisted of 29 primary hybrids and 12 subclones; 12 were positive. The 44 human–mouse hybrids contained 16 primary clones and 28 subclones; 15 were positive.