TABLE 3.
TWO-POINT LOD SCORES FOR hPPARγ VERSUS OTHER LOCI ON CHROMOSOME 3p22-p26
| Locus† | Z at Ө* | Өmax | Zmax | Confidence Interval‡ | Physical Location | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 0.05 | 0.1 | 0.2 | 0.3 | 0.4 | |||||
| D3S191 | − ∞ | 3.6 | 5.2 | 5.2 | 3.9 | 2.1 | 0.143 (0.092,0.196) |
5.5 (5.7) |
0.077–0.255 | pter-p25 |
| D3S18 | − ∞ | 19.5 | 20.6 | 18.1 | 13.0 | 6.5 | 0.096 (0.048,0.143) |
20.6 (21.3) |
0.055–0.139 | p26-p25 |
| RAF1 | − ∞ | 17.1 | 16.8 | 13.6 | 9.0 | 4.0 | 0.065 (0.056,0.071) |
17.2 (17.2) |
0.040–0.118 | P25 |
| D3S154 | − ∞ | 7.9 | 7.6 | 5.4 | 2.9 | 0.8 | 0.059 (0.119,0.001) |
8.0 (8.1) |
0.020–0.153 | p25 |
| D3S588 | − ∞ | 11.6 | 15.8 | 15.7 | 11.7 | 6.1 | 0.142 (0.136,0.146) |
16.5 (16.5) |
0.097–0.203 | p24 |
| THRB | − ∞ | 4.6 | 9.1 | 10.0 | 7.5 | 3.8 | 0.162 (0.116,0.213) |
10.3 (10.6) |
0.109–0.229 | p24.1-p22 |
The LOD scores (Z) at selected recombination fractions (Ө) are shown. The most likely recombination fraction (Өmax) and LOD scores (Z max) are also shown. [All values are computed assuming no sex difference in recombination frequencies except values in parentheses, which give sex-specific (male-female order), recombination fractions, and Z max]
Probe-enzyme combinations for these loci are: D3S191 = 38-96 with Msp I; D3S18 = L162 with Bam HI and Dra I enzymes combined as haplotype; RAF1 = = p628 with Taq I and Bgl I enzymes combined as a haplotype; D3S154 = LIB34-60 with Bg1 II;D3S588 = LIB12-69 with Hind III; THRB = pBH302 with Hind HIII.
Confidence intervals for recombination fractions over a 10-fold range of likelihood.
Technical comments: Several errors were detected and corrected. The genotypes for paternal grandparents 133311 and 133312 with L162/Dra I were deleted due to incompatibility. Genotypes for individuals 1204, 1208, 1703, and 1704 were omitted from the D3S18 haplotype because these individuals were recombinant between genotypes with Bam HI and Dra I. Individuals 6605 and 133208 were recombinant between RAF1/Bg1 II and RAF1/Taq I, and these individuals were deleted from the RAF1 haplotype. Finally, a phase error was detected in CEPH family 1420 between hPPARγ (PPARG) and both RAF1 and THRB. No errors could be found on reexamination of the autoradiographs for hPPARγ/Xba I in this family. This phase error was eliminated by the conservative change involving deletion of genotypes for paternal grandparents 142009 and 142010 for hPPARγ/Xba I.
Genotypes for all loci except PPARG were contributed to the CEPH data base by the laboratory of Dr. B. Zbar.