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. 2017 Jul 6;313(4):G320–G329. doi: 10.1152/ajpgi.00121.2017

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Fig. 3. — Intracisternal injection of RX-77368 abolished abdominal surgery (AS)-induced increase in the number of MHCII⁺/CD206⁻ (M1 like) macrophages in the gastric myenteric plexus assessed 6-h postsurgery. Top: double staining of MHCII (red) and CD206 (green) in rats treated with intracisternal saline (10 µl, −1 h)/sham operation; intracisternal saline/AS; intracisternal RX-77368 (50 ng/rat, −1 h)/sham operation; and intracisternal RX-77368 (50 ng/rat, −1 h)/AS. Bar = 100 µm. Bottom: quantitative analysis of macrophages counted in 20 optical fields/rat (475 × 475 μm/field) with each field containing a ganglium. M1, MHCII⁺/CD206⁻; M2, MHCII⁻/CD206⁺. Each column represents means ± SE of no. of rats indicated at the bottom. *P < 0.05 vs. M1 in saline/sham; #P < 0.05 vs. M1 in saline/AS group; +P < 0.05 vs. M1 in RX-77368/sham group; &P < 0.05 vs. M1 in RX-77368/AS group.