Fig. 1.

OsbHLH035 expression is induced by salt and is abolished in the NG7221 line. a The structure of the OsbHLH035 gene and the retrotransposon insertion site. b OsbHLH035 expression patterns in aerial tissues from third-leaf-stage WT and NG7221 seedlings. The seedlings were grown on basal medium for 13 days and then transferred to basal medium containing 0 (CK) or 250 mM NaCl for an additional day. The primer positions and sequences are shown in (a) and Additional file 2: Table S1. Arabic numerals in (b) represent three independent biological replicates within each genotype