Fig. 3.

Validation of differentially expressed circRNAs in NJCMS1A and NJCMS1B by qRT-PCR. The x-axis represents the names of circRNAs, while the y-axis represents the circRNA relative expression level generated from qRT-PCR analysis and high-throughput sequencing. Expression of the GADPH gene was used as the internal reference. All qRT-PCR reactions were performed with three biological replicates, and the error bars indicate the standard errors of the means of 2^–ΔΔCt, with NJCMS1B as a control