Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 12;20:112. doi: 10.1186/s13058-018-1028-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Nogo-B receptor (NgBR) is highly expressed in the tamoxifen resistant MCF-7-TamR and T47D-TamR cells. a Colony formation assay was performed as described in “Methods”. Wild-type MCF-7 and tamoxifen-resistant MCF-7-TamR cells were treated with different concentrations of 4-OHT (0, 1 and 5 μM). b Quantification of colony number presented in colony formation assays of MCF-7 and MCF-7-TamR cells. c Colony formation assay of wild-type T47D and tamoxifen-resistant T47D-TamR cells treated with different concentrations of 4-OHT (0, 1 and 5 μM). d Quantification of colony number in colony formation assays of T47D and T47D-TamR cells. e, f mRNA level of NgBR was increased in MCF-7-TamR and T47D-TamR cells as compared to wild-type MCF-7 and T47D cells, respectively. The relative amount of NgBR mRNA level was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). g NgBR protein level was increased in MCF-7-TamR cells. Protein levels of Nogo-B, epidermal growth factor receptor (EGFR), human epidermal growth factor receptor 2 (HER2), estrogen receptor alpha (ERα), p53 and survivin in MCF-7 and MCF-7-TamR cells were determined using western blot analysis. h Quantitative analysis of protein levels using ImageJ and normalized to the housekeeping gene β-actin. Data are presented as fold changes in MCF-7-TamR cells compared to MCF-7 cells. The data are from three separate repeat experiments, and are presented as the mean ± SD (*p < 0.05, n = 3)