Table 2.

Detailed comparison of LAMP and nested PCR combined with restriction enzyme digestion for Plasmodium identifications

Parasite(s) detected by each methoda (no. of samples; n = 3650 blood samples)
LAMP	Nested PCR combined with RE digestion of LAMP productsb
P. falciparum (27)	P. falciparum (26), negative (1)c
P. vivax (47)	P. vivax (45), negative (2)d
P. falciparum + P. vivax (4)	P. falciparum + P. vivax (4)
P. malariae (2)	P. malariae (2)
Plasmodium spp.e (8)	Plasmodium spp. (8)
Negative (3562)	Negative (3562)

^aEach row displays the results obtained from identical blood samples. Discordant results between LAMP and nested PCR combined with restriction enzyme digestion are showed in italicface

^bGenus and species-specific LAMP products were digested with restriction enzyme to confirm the specificity of LAMP products

^cPositive for P. falciparum by LAMP but negative after restriction enzyme digestion of LAMP products

^dPositive for P. vivax by LAMP but negative after restriction enzyme digestion of LAMP products

^ePositive for genus-specific LAMP but negative for species-specific LAMP