Fig. 1.

Phylogenetic tree based on the amino acid sequences of 77 chalcone synthase proteins. Among these sequences, 9 (XM_006487413.1, XM_006487414.2, XM_006472682.2, XM_006489733.1, NM_001320057.1, NM_001320057.1, XM_006489733.1, XM_006472682.2) were derived from NCBI, 16 (CICLE_v10001405 m, CICLE_v10001413 m, CICLE_v10003127 m, CICLE_v10005133 m, CICLE_v10015535 m, CICLE_v10028604 m, CICLE_v10028605 m, CICLE_v10030093 m, CICLE_v10030398 m, orange1.1 g016081 m, orange1.1 g016094 m, orange1.1 g016111 m, orange1.1 g016330 m, orange1.1 g036844 m, orange1.1 g044410 m, orange1.1 g047978 m) were derived from Phytozome and the remaining 52 were derived from the Orange Genome Annotation Project. The red triangle represents the selected candidate sequences used for the expression analysis. The alignments were saved and executed using MEGA version 7.1 to generate a neighbour-joining tree with a bootstrapping (1000 replicates) analysis, and gaps were addressed via pairwise deletion