Fig. 7.

Overexpression of the CitCHS2 gene in citrus plants

a:1428 bp fragment containing the CitCHS2 gene ORF was constructed in the pBI121 vector. b: 1428 bp fragment was cloned via RT-PCR. M, marker; 1, 1428 bp fragment contain the ORF of CitCHS2. c: Pattern of double digestion by XbaI and SacI of the p-EASY vector containing the 1428 bp fragment and the pBI121 vector. M, marker; 1, the p-EASY vector containing the 1428 bp fragment; 2, the pBI121 vector. d:Validation of the constructed pBI121 and CitCHS2 ORF vector via double digestion by XbaI and SacI. M, marker; 1, the constructed pBI121 vector. e: Colony PCR of the positive Agrobacterium tumefaciens strain EHA 105 that contains the constructed pBI121 vector. M, marker; 1, the positive colony PCR product. f: Callus generated from the epicotyl of C. sinensis after the infection of the Agrobacterium tumefaciens. g: Grafted positive plants grown on the rootstock