Fig. 2.

Drug-induced alterations in gene expression in the mouse NAc and PFCx. Hierarchical clustering analysis of drug-induced changes in gene transcript levels. RNA-seq results are shown as a heat map and include 113 transcripts with a genome-wide significance (FDR < 0.001 two-way ANOVA of the treatment factor). Transcriptional events regulated by the drugs are listed in Additional file 3. Colored rectangles represent the transcript abundance 2 h after the injection of the drug indicated above the rectangle (HAL: haloperidol; RIS: risperidone; MIA: mianserin; VEN: venlafaxine; MET: methamphetamine; KET: ketamine and SAL: saline control). Representative genes are presented on the right. The intensity of the color is proportional to the standardized value (z-score between − 2 and 2) of each RNA-seq measurement, as indicated on the bar below the heat map. Clustering was performed using Euclidean distances. Major branches from the clusters of drug-responsive changes are labeled 1–4