Table 2.
Phosphorylated hepatic proteins with correlations with fibrosis and a higher percentage of collagen deposition in livers of NAFLD patients
| Endpoint | Protein intensity values (mean) | ||
|---|---|---|---|
| MITOGENESIS | Fibrosis (stage ≥ 2)a,b | Fibrosis (stage < 2)a,b,c | Percentage collagen ρc,d |
| RTKS and LIGANDS | |||
| c-Kit Y703 | 12,023.3253 | 8509.7594** | 0.2727* |
| EGFR Y1045 | 7752.7248 | 5367.3954** | 0.3061* |
| ErbB2 HER2 Y1248 | 13,638.4722 | 10,163.0936** | 0.2544* |
| Met Y1234/1235 | 8394.0971 | 5442.5366*** | 0.4082*** |
| Ret Y905 | 4840.5597 | 2796.3384** | 0.2564* |
| Ron Y1353 | 11,455.9458 | 8217.8116** | 0.2564* |
| DOWNSTREAM SUBSTRATES | |||
| CrkII Y221 | 11,296.7507 | 7283.2336** | 0.2844* |
| c-Myc | 16,583.2182 | 13,624.0484* | 0.2705* |
| ERK 1/2 TOTAL | 27,683.0657 | 22,584.3185** | 0.3574** |
| FRS2 alpha Y436 | 6745.9092 | 4569.8235* | 0.3382** |
| IRS 1 S612 | 6603.2819 | 4223.5915* | 0.2756* |
| PKA C T197 | 13,849.3962 | 11,729.8932* | 0.2968* |
| Src Family Y416 | 3488.0194 | 1874.4209** | 0.2631* |
| SURVIVAL | |||
| AKT T308 | 19,966.2896 | 11,089.2265** | 0.3763** |
| AKT TOTAL | 15,739.0600 | 10,127.1820** | 0.3282** |
| ASK1 S83 | 2072.4277 | 1209.4175** | 0.2563* |
| Ephrin A3 Y779/A4 Y779/A5 Y833 | 10,051.2853 | 9008.6468* | 0.3179* |
| PDK1 S241 | 13,526.9204 | 10,692.6456** | 0.2990* |
| PI3K p85 Y458 p55 Y199 | 5930.4957 | 4023.6630** | 0.4243*** |
| PTEN | 26,544.6698 | 22,904.4300* | 0.2628* |
| PTEN S380 | 12,498.2321 | 7720.4331*** | 0.2677 |
| INFLAMMATION/IMMUNE FUNCTION | |||
| Zap 70 Y319/Syk Y352 | 8322.9034 | 4839.8932*** | 0.3189* |
| cPLA2 S505 | 11,697.1946 | 8108.4437** | 0.2861* |
| PLCgamma1 Y783 | 11,995.7606 | 8488.3315** | 0.2824* |
| Stat4 Y693 | 8407.7191 | 5859.6088** | 0.3010* |
| Stat6 Y641 | 7840.7122 | 4793.4883*** | 0.3226* |
| Tyk2 Y1054/1055 | 8760.8030 | 5060.9800*** | 0.2955* |
| p38 MAPK T180/Y182 | 8093.9200 | 6807.1800 | 0.3901** |
| APOPTOSIS | |||
| Bak | 20,974.0624 | 18,147.0249** | 0.3262** |
| Survivin | 11,593.7421 | 8882.5924*** | 0.3180* |
| MOTILITY AND CELL ADHESION | |||
| Cofilin S3 | 11,887.6509 | 2171.5929*** | 0.3369** |
| FAK Y576/577 | 7300.4566 | 5303.6335* | 0.3382** |
| OTHERS | |||
| ALDH | 21,453.9429 | 26,006.4687** | −0.3009* |
| IL-10 | 1731.1300 | 1311.5200 | 0.5539*** |
a Proteins in liver tissue, detected using RPMA, that were expressed at higher and significant level in patients with fibrosis ≥ 2 compared with fibrosis < 2
b Non-parametric Kruskal–Wallis H test was used to identify the significantly differentially expressed proteins between the two groups
c Protein level correlation with % collagen tested using Spearman rank correlation test
d Correlation coefficient (ρ) describes the strength and direction of association between proteins and % collagen
p values < 0.05 are considered significant; *p < 0.05, **p < 0.01, ***p < 0.001