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. 2018 Aug 6;9(17):3067–3077. doi: 10.7150/jca.26382

Figure 2.

Figure 2

The TAD domain of NKX2-2 is essential for its inhibitory effects on migration and invasion. (A) Schematic showing the FLAG-tagged NKX2-2 constructs. The positions of the transcriptional repressor domain (TN), the NK2-specific domain (SD) and the transcriptional activation domain (TAD) are shown. (B) Western blot analysis of 143B cells infected with the indicated cDNA constructs (C, D) The migration of 143B cells transduced with indicated cDNA constructs was determined as described in the “Methods”. Representative images (100×) of the indicated stably transfected cell lines are shown (C). n = 5 fields per replicate; 3 replicates per condition, data are representative of three independent experiments (D). (E, F) The invasion of 143B cells transduced with the vector, NKX2-2 or NKX2-2 ΔTAD was determined as described in (C, D). (G, H) The migration and invasion of U2OS cells transduced with the vector, NKX2-2 or NKX2-2 ΔTAD were determined as described in (C, D). Data are presented as the means ± SDs. Two-tailed unpaired t test: **P< 0.01 and ***P < 0.001, ns: not significant.