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. 2018 Aug 6;9(17):3149–3155. doi: 10.7150/jca.25395

Figure 1.

Figure 1

THZ1 induced C2C12 cell apoptosis and inhibits C2C12 cell proliferation. (A)C2C12 cells were treated with gradient concentration of THZ1 (0,200,400,600,800 nM) for 24h.The percentage of live and apoptosis cells were measured on flow cytometry.(B)Independent experiment was repeated for three times. Bar graph is for quantification of figure 1A.(C) C2C12 cells were treated with gradient concentration of THZ1 (0,100,200,400,800 nM) for 24h.Intracellular ROS were measured on flow cytometry.(D) Independent experiment was repeated for three times. Bar graph is for quantification of figure 1C.(E)OD values in MTT assay were measured after C2C12 cells were treated with gradient concentration of THZ1 (0,25,50,100,200,300,400,600 nM) for 24h,48h,72h respectively.(F)C2C12 cells were incubated for 24h in different concentration (0,50,100,150,200 nM) and cell cycle was determined on flow cytometry. (#P>0.05, *P<0.05, **P<0.01, ***P<0.001, n=3, bars represent SD).