Figure 2.

THZ1 inhibited migration of C2C12 cells. (A)Six scratch wounds were made per disk when cell density reached 60%. Growing medium was changed with medium of 3% FBS and 1% penicillin and C2C12 cells were treated with gradient concentration (0, 50,100 nM). Pictures were taken in 12 h, 24 h, 48 h respectively, among which pictures of C0 were taken immediately after the scratch was created.(B)(C)(D) Area taken by cells inside the two black lines was calculated by image. Nine cross points each picture was put into calculation and independent experiment was repeated for three times. (*P<0.05, **P<0.01, n=3, bars represent SD).