Figure 4.

Intraperitoneal injection of BM-derived HSPCs contributes to the emergence of innate lymphoid-like cells in the peritoneal cavity of low-dose zymosan-injected mice. (A) Total bone marrow cells from congenic CD45.1 mice underwent magnetic and electronic cell separation in order to get pure hematopoietic stem cells (LSK). First, cells were selected based on the positive expression of CD117 (c-kit) and then stained for Lineage and Sca-1. A population of Lineage⁻c-kit⁺Sca-1⁺ was finally electronically sorted out. (B) CD45.2 C57BL/6J mice were injected intraperitoneally with a low-dose of zymosan and 4 h later the same recipients got 30,000 of LSK cells (i.p injection). Twenty-four hours post-injection, cells were recovered from the peritoneal cavity of injected mice and stained for several markers of mature immune cells (including CD3e, CD19, CD11b, F4/80, CD11c, and Ly6c) or markers for innate lymphoid-like cells such as Lineage, Sca-1 and CD127. (One representative experiment is shown from 3 independent ones).