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. 2018 Sep 5;9:2007. doi: 10.3389/fimmu.2018.02007

Figure 5.

Figure 5

Treatment with Alpha-1-Antitrypsin inhibits the emergence of total ILCs in the peritoneal cavity of zymosan-injected mice. (A) C57BL/6J mice were injected intraperitoneally with low- or high-dose of zymosan and AAT treatment 15 min later. Peritoneal fluid from both zymosan-treated and PBS-injected (control) mice was purified and stained for markers of ILCs such as CD45, Lineage, CD127, and CD90. One representative flow cytometry analysis is shown from 4 independent experiments (n = 4). (B) Cells were stained for Lineage, CD127, CD25, Sca-1, and KLRG-1 in order to identify bone marrow-derived ILC2 and ILC2P cells (n = 4). (C) Statistical analysis of the frequency of Lineage−CD127+CD90+ cells (n = 4). (D) Flow cytometry gating strategy for ILC2 and their progenitor cells (ILC2P) in both mice injected with low- and high-dose of zymosan after treatment with AAT (n = 4). (E) Statistical analysis for the frequency of Sca-1+CD25+ cells within CD45+Lineage− cells (n = 4). **p < 0.005.