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. 2017 Jan 26;6:1–8. doi: 10.1016/j.reth.2016.12.002

Fig. 2.

Fig. 2

Development of GlycoStem-HP. (A) Cell culture supernatants of 201B7 hiPSCs were serially diluted with DMEM containing 2% FBS and reacted with rBC2LCN immobilized on a microtiter plate. The captured rBC2LCN-positive podocalyxin was detected with HRP-labeled R-10G or rABA. (B) The captured rBC2LCN-positive podocalyxin was detected with HRP-labeled SSEA3, SSEA4, Tra-1-60, Tra-1-81, or anti-podocalyxin pAb. Absorbance at OD450 was measured. Data shown are the mean ± SD of triplicate samples. (C) Effect of FBS on GlycoStem-HP. Cell culture supernatants of 201B7 hiPSCs were serially diluted with DMEM containing different percentages (0–20%) of FBS and reacted with rBC2LCN immobilized on a microtiter plate. The captured rBC2LCN-positive podocalyxin was overlaid with HRP-labeled R-10G. Absorbance at OD450 was measured. Data shown are means of triplicate samples.