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. 2017 Jan 26;6:1–8. doi: 10.1016/j.reth.2016.12.002

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© 2017, The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Monitoring of the number of hiPSCs during cardiomyocyte differentiation using GlycoStem-HP. Supernatants from 253G1 hiPSC cultures during cardiomyocyte differentiation were analyzed by GlycoStem-HP. The apparent cell number was calculated from the linear equation obtained from the standard curve generated using cell culture supernatants of 253G4 hiPSCs and expressed as AU (closed circles). The number of total cells (open squares) and Tra-1-60/rBC2LCN-positive hiPSCs (open triangles) determined by flow cytometry are also shown.