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Detection of fetal hNSCs and hiPSC-derived hNSCs. (A) Supernatants from Ff-I01 hiPSC cultures were serially diluted with PBS and analyzed by GlycoStem-HP. (B) Cell culture supernatants of fetal hNSCs and hiPSC-derived hNSCs were serially diluted with PBS and analyzed by GlycoStem-HP. Absorbance at OD450-620 was measured. Data are shown as the mean ± SD of triplicate samples. (C) hiPSC-derived hNSCs were stained with Tra-1-60 and SSEA4 and analyzed by flow cytometry.
