Fig. 7.
Detection of undifferentiated hiPSCs residing among hiPSC-derived hNSCs. (A) A standard curve was generated using the conditioned medium obtained from 24 h culture of Ff-I01 hiPSCs (1.5 × 105) serially diluted with StemFit®AK03 medium. (B) hiPSC-derived hNSCs (1.5 × 105 cells) were then cultured in the presence of 750 (corresponding to 0.5% hiPSCs), 150 cells (0.1% hiPSCs), or 75 cells (0.05% hiPSCs) of Ff-I01 hiPSCs in 1 mL of cell culture media for 24 h. The cell culture supernatants were recovered and then analyzed by GlycoStem-HP. The seeding cell number (cells/mL) and the apparent cell number (AU) of hiPSCs estimated by GlycoStem-HP were shown in filled and open bar graphs, respectively. Data of GlycoStem-HP (open bar graph) are shown as the mean ± SD of triplicate samples.