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. 2018 Aug 7;8(16):4409–4428. doi: 10.7150/thno.26467

Figure 1.

Figure 1

Knock-down (KD) of Chi3L1 inhibits lung metastasis of B16F10 melanoma in C57BL/6 mice model. (A) Gene (Chi3L1)-disease network was analyzed based on the GWAS/OMIM/DEG records (p<10-6). (B) Image of lung tumor tissues of C57BL/6 mice injected with B16F10 mouse melanoma. Black colonies are B16F10 melanoma colonies on the lung tumor tissue surface. (C) Counts of total and >2 mm diameter nodules in each lung tumor tissue. *, p<0.05. Error bars represent standard deviation. Normal mice, n=6; Chi3L1 KD mice, n=6. (D) Image of lung tumor tissue section stained with hematoxylin and eosin. (E) Immunohistochemistry to detect Chi3L1-positive cells in mice lung tissues (blue denotes nuclei; brown denotes Chi3L1 expression). (F) Expressions of Chi3L1, PCNA, MMP-9, MMP-13, and VEGF in C57BL/6 mice lung tissues (n=5 mice). Values below the Western blot bands indicate densitometry analysis using ImageJ software.