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. 2018 Aug 7;8(16):4409–4428. doi: 10.7150/thno.26467

Figure 3.

Figure 3

Up-regulated USF1 expression via knock-down (KD) of Chi3L1 in vivo and in vitro. (A) Relative expressions of Chi3L1, USF1, SPI1, SP3, APOE, and MMP-9 mRNA levels in Chi3L1 KD mice compared to normal mice. *, p<0.05. Lower panel shows expression of USF1 protein in lung tumor tissues of Chi3L1 KD mice. (B) Expression of USF1 mRNA (upper panel) and protein (lower panel) in lung tumor tissues of Chi3L1 KO mice (n=3 in each). Quantification of protein expression was measured and analyzed using ImageJ software. *, p<0.05. (C) Relative expressions of Chi3L1, USF1, SPI1, SP3, APOE, and MMP-9 mRNA in A549 cells by siChi3L1 treatment compared to siControl. *, p<0.05. (D) Relative expressions of Chi3L1, USF1, SPI1, SP3, APOE, and MMP-9 mRNA in H460 cells following siChi3L1 treatment compared to siControl. *, p<0.05. (E) Expression of USF1 protein with siChi3L1 treatment in A549 and H460 cell lines. (F) Relative expressions of USF1 mRNA following rhChi3L1 treatment. *, p<0.05. (G) Expressions of Chi3L1 and USF1 after rhChi3L1 treatment in A549 and H460 cell lines. (H) USF1 transcriptional activity following rhChi3L1 treatment or siChi3L1 treatment in A549 and H460 cell lines. ERK inhibitor (U0126 10 μM). *, p<0.05; **, p<0.01; #, p<0.05; ##, p<0.01. All in vitro experiments were prepared in duplicate and repeated three times. Values below the Western blot indicate densitometry analysis using ImageJ software.