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. 2018 Jul 30;8(16):4321–4331. doi: 10.7150/thno.26509

Figure 2.

Figure 2

Effects of Shh gene delivery on the function and histology of irradiated parotids. (A) Stimulated parotid saliva flow rates from the irradiated side or the NT side of animals with no further treatment or of animals who received delivery of Ad-GFP or Ad-Shh 4 or 16 weeks after IR into irradiated parotids were measured separately every two weeks from two weeks before IR until 20 weeks after IR. Percentages of saliva flow rate from the irradiated parotid to that from the NT parotid of the same pig are shown. (B) The wet weights of irradiated parotids collected 20 weeks after IR were normalized to that of NT parotid of the same pig. (C-D) Parotids collected 20 weeks after IR were examined by H&E staining and acinar areas were quantified from 30 fields from 3 independent samples (n=30). (E-F) Parotids collected 20 weeks after IR were examined by Masson's trichrome staining and the blue-stained fiber areas were quantified (n=30). (G) The expression of acinar markers AQP5 and Chrm3 in parotids collected 20 weeks after IR were examined by qRT-PCR (n=3).