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. 2018 Jul 30;8(16):4321–4331. doi: 10.7150/thno.26509

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Effects of Shh gene delivery on the IR-induced cellular senescence. 5 weeks after IR, we collected parotids not irradiated (NT), irradiated alone (IR), and transduced with Ad-GFP or Ad-Shh 4 weeks after IR for the following assays. (A) The activity of senescence marker SA-β-gal (blue) in parotids was clearly increased by IR compared to the marginal activity in NT samples, and this increase was inhibited by Ad-Shh but not Ad-GFP delivery 4 weeks after IR. (B-C) IHC for the proliferation marker Ki67 indicated that Ki67 expression was significantly decreased by IR compared to the NT samples, and Ad-Shh but not Ad-GFP delivery 4 weeks after IR preserved Ki67 expression. (D) qRT-PCR analysis (n=3) indicated that the mRNA expression of senescence marker p21/CDKN1A was significantly increased by IR compared to NT samples, and this increase was significantly inhibited by Ad-Shh but not Ad-GFP delivery 4 weeks after IR.