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. 2018 Jul 30;8(16):4321–4331. doi: 10.7150/thno.26509

Figure 5.

Figure 5

Effects of Shh gene delivery on the IR damage to parasympathetic innervation in parotids. (A-B) In parotids collected 20 weeks after IR, IF staining of parasympathetic innervation marker ACHE and quantification of ACHE signals indicated that parasympathetic innervation was impaired by IR but preserved by Ad-Shh transfer 4 weeks after IR, whereas Ad-Shh transfer 16 weeks after IR and Ad-GFP transfer at either time point did not significantly preserve ACHE expression (n=30). (C-D) IHC staining and quantification of cholinergic receptor muscarinic 1 (Chrm 1) in parotids 20 weeks after IR indicated that the Chrm1 expression was downregulated by IR but preserved by Ad-Shh transfer 4 weeks after IR, whereas Ad-Shh transfer 16 weeks after IR and Ad-GFP transfer at either time point did not significantly preserve Chrm1 expression (n=30). (E-F) Western blot and quantification of Chrm1 confirmed the IHC data (n=3). (G-H) In parotids collected 5 weeks after IR, qRT-PCR assays indicated that the mRNA expression of BDNF and NRTN was decreased by IR alone or IR plus GFP gene transfer, but was preserved by Shh gene transfer 4 weeks after IR (n=3).