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. 2018 Aug 7;8(16):4429–4446. doi: 10.7150/thno.26717

Figure 4.

Figure 4

CAQK-LIP-GFs/DTX@HP promotes axonal generation and re-myelination essential for locomotion. (A) Representative images containing astrocytic and neurofilament (NF-200) immunofluorescence on spinal cord sections at 28 days after SCI. Scale bar = 150 μm. (B-C) Quantitative analysis of average distance of NF-200 positive axon from lesion margin and NF-200 positive axon area in the lesion. N= 3. (D) The expression of biotin dextran amine (BDA) protein in the immunofluorescence staining at different positions of the spinal cord on the 28th day after SCI. Scale bar = 50 μm. (E) Beta-3 tubulin (Tuj-1) immunolabeling of neurons with or without inhibitory chondroitin sulfate proteoglycans (CSPGs, 3.34 µg/mL) substrates at DIV5 (days in vitro for 5 days). Scale bar = 50 μm. (F-G) Neurite length of cortical neurons at DIV5 under indicated conditions. N= 4. (H-I) Western Blot (WB) protein expressions and quantification data of MBP in each group. N= 4. (J-K) Representative images of NF-200 (green) and myelin basic protein (MBP) immunofluorescence on spinal cord sections at 28 days after SCI. Scale bar = 50 μm. (L) Transmission electron microscopy images showing the microstructure of the myelin sheath in each group. *P < 0.05, **P< 0.01, ***P< 0.001.