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. 2018 Aug 7;8(16):4429–4446. doi: 10.7150/thno.26717

Figure 5.

Figure 5

CAQK-LIP-GFs@HP reduced apoptosis and promoted rehabilitation of neurons by impacting the inflammatory reaction and macrophage polarization. (A) Immunofluorescence staining for terminal deoxynucleotidyl transferase (TdT) dUTP nick end labeling (TUNEL, green) of sections from the injured spinal cord in each group. Scale bar = 50 μm. (B) Quantitative estimation of apoptotic and TUNEL-positive cells from seven independent sections within 5 mm of the injury epicenter. (C-D) Expressions and quantification data of C-caspase-3 from each group. Scale bar = 20 µm. N= 3. (E-G) Protein expressions and quantification data of nestin and growth-associated protein 43 (GAP-43) in each group. N= 4. (H) Immunofluorescence images of the spinal cord show the relationship of astrogliosis (red) and monocytic phagocytes (CD68, green) in each group. Scale bar = 750 µm (A-B); scale bar = 150 µm (A1-D2). (I-K) fluorescence intensity quantification of CD68 in different locations. N= 3. (L-N) Protein expressions and quantification data of CD206 and CD86 in each group. N= 3. *P < 0.05, **P< 0.01.