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. 2018 Aug 7;8(16):4462–4476. doi: 10.7150/thno.24395

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(A) Schematic overview of the design of the dimeric conjugate and the mode of binding of the biparatopic affibody to VEGFR2, blocking the VEGF epitope. (B) Purity determination by analytical RP-HPLC for Z_VEGFR2-Bp₂. (C) LC-ESI/MS mass spectrum for Z_VEGFR2-Bp₂. The experimentally determined mass is indicated in the spectrum. (D) Determination of refolding capacity. CD spectra before and after heat treatment to 90 °C. (E) Determination of heat stability. CD ellipticity at 221 nm as a function of temperature.