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DNA volume decreases in response to antibiotic-induced stress. (A) Deconvolved widefield fluorescence microscopy images of lipid (Nile Red) and DNA (DAPI) patterns in (un)treated M. smegmatis mc2 155. Deconvolved DAPI signal was used to compute DNA volume. Scale bar represents 1 μm. (B) Quantification of DNA volume. Bars represent mean ± standard deviation of 3 independent experiments (n = 60 bacteria per condition). ***P < 0.0005.
