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. 2018 Sep 5;9:2034. doi: 10.3389/fmicb.2018.02034

Figure 7.

Figure 7

Inhibition DNA acetylation after condensation DNA, improves killing M. smegmatis. (A) Fluorescence microscopy of DNA of M. smegmatis treated with FA, compound EIS 1a*, inhibiting the Eis enzyme (Eis) or FA and subsequently Eis inhibitor (FA → EIS), the reverse order (EIS → FA) or control (Con) (Nile Red stained as counterstaining is presented in Supplemental Figure 5A). (B) Similar set-up as in (A) imaged after 2 days of incubation (Nile Red stained as counterstaining is presented in Supplemental Figure 5B). (C) Relative Colony Forming Units (CFU) based on untreated controls at 6 days after incubation in liquid antibiotic containing medium and plated on antibiotic free plates (relative CFU is calculated based on untreated controls and average of 3 independent experiments with standard error and * for significant differences to control P < 0.05). (D) Representative colonies from 10 μl 7H9 medium at a dilution of 102, 104, or 105 with FA, Eis inhibitor or antibiotic combinations or control after incubation for 6 days in 7H9 and subsequently grown on 7H10 plate without antibiotics. Bars represent 1 μm.