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. Author manuscript; available in PMC: 2019 Jan 30.
Published in final edited form as: Nat Plants. 2018 Jul 30;4(8):586–595. doi: 10.1038/s41477-018-0213-y

Figure 2.

Figure 2

The identity of the sub-epidermis before and after CIF2 treatment. (A-L) Confocal images of fixed and stained roots. a, Cortex marker p315.1::erGFP in Col-0 (WT), b, pWER::SHR-GFP roots without CIF2 and c, with CIF2. Cortex marker pCO2::H2B-YFP in d Col-0, e pWER::SHR-GFP roots without CIF2 and f with CIF2. SGN3 in f Col-0, h pWER::SHR-GFP roots without CIF2 and i with CIF2. Maximum projections of MYB36 transcriptional reporter in j Col-0 (live sample), k pWER::SHR-GFP roots without CIF2 and l with CIF2. CW = Calcofluor white, PI = propidium iodide. White asterisks indicate epidermis cell files. Red asterisks indicate sub-epidermis cell file. Red arrowheads in g-l indicated ectopic expression. White arrowheads in g-l indicate endogenous expression. Scale bars = 20 μM. For a-l, n ≥ 10 roots. m, Schematic of the two genotypes and two treatments used for FACS. Cells with CASP1 (yellow) were enriched for by cell sorting. n, Heat map of the mean Index of cell identity value of three replicates from samples described in m compared to transcriptome from RootMap 3.0 (cell-types listed along bottom, see Table S2 for RootMap 3.0 libraries and values). Black asterisks indicate ICI p-value <0.05. Each experiment was repeated at least three times.