Table 1.
Effect of p5RHH-JNK2 siRNA nanoparticles on blood chemistry and counts
| Test | HBSS (mean±SD) | JNK2 siRNA NP* (mean±SD) |
|---|---|---|
| RBC (mL/mm2) | 9.81±1.51 | 9.53±0.76 |
| Hemoglobin (g/dL) | 13.70±2.13 | 13.65±1.08 |
| Hematocrit HC (%) | 42.33±7.26 | 43.40±2.74 |
| Mean corpuscular volume (μm3) | 43.13±1.52 | 45.60±1.12 |
| Mean corpuscular hemoglobin (pg) | 14.00±0.44 | 14.33±0.05 |
| Mean corpuscular hemoglobin concentration (g/dL) | 32.43 ±0.50 | 31.43±0.74 |
| Platelets (thsn/cu mm) | 1,151.33±20.60 | 1,113.75±69.31 |
| BUN (mg/dL) | 23.75±3.33 | 27.50±0.71 |
| Creatinine (mg/dL) | 0.86±0.29 | 1.01±0.31 |
| AST (U/L) | 102.38±41.76 | 99.00±29.82 |
| ALT (U/L) | 50.57±9.03 | 39.75±10.72 |
| Total protein (g/dL) | 10.24±2.65 | 9.86±3.04 |
| Glucose (mg/dL) | 283.63±30.09 | 214.50±48.79 |
| Sodium (mmol/L) | 144.80±3.3 | 145.20±2.5 |
| Potassium (mmol/L) | 4.6±1.1 | 4.5±0.5 |
| Chloride (mmol/L) | 118.6±13.3 | 117.2±12.0 |
Notes:
*
After intravenous injection of seven doses of JNK2 siRNA nanoparticles (0.5 mg siRNA/kg) over 23 days. Blood counts: HBSS n=3 and p5RHH-JNK2 siRNA nanoparticles n=4. Kidney function: HBSS n=8 and p5RHH-JNK2 siRNA nanoparticles n=6. Liver function: HBSS n=8 and p5RHH-JNK2 siRNA nanoparticles n=3. Glucose: HBSS n=8 and p5RHH-JNK2 siRNA nanoparticles n=5. Blood electrolytes: HBSS n=5 and p5RHH-JNK2 siRNA nanoparticles n=5.
Abbreviations: HBSS, Hanks’ Balanced Salt solution; HC, hematocrit; NP, nanoparticles; RBC, red blood cells.