Figure 2.

Analysis of the effect of polyelectrolyte nanocapsules on human PBMCs and erythrocytes.

Notes: (A) Viability of PBMCs. PBMCs were incubated for 48 hours with 1.5 mM NaCl or with different nanocapsules (~2×10¹¹/mL). All cells and dead cells were counted. (B) Detection of nanomaterial-induced hemolysis. RBCs were exposed for 3 hours to 1.5 mM NaCl or different nanocapsules or Triton X-100. Detection of hemoglobin was performed spectrophotometrically. Morphological changes were analyzed using inverted light microscope. Arrows indicate cell aggregates or cells with changed morphology. *P<0.05.

Abbreviations: NC5, five-layer nanocapsules; NC5-PEG, five-layer PEGylated nanocapsules; NC6, six-layer nanocapsules; PBMC, peripheral blood mononuclear cell; PEG, polyethylene glycol; RBC, red blood cell.