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. 2018 Aug 16;11(3):828–841. doi: 10.1016/j.stemcr.2018.07.007

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Identification of Positive and Negative Cell-Surface Markers Specific to hESC-Derived Ventricular Cardiomyocytes

(A) After exclusion of debris and doublets based on light scatter properties, single cells were analyzed for the expression of MYL2-GFP. The gating strategy for the detection of MYL2-GFP⁺ cells was defined using parental H9 cells as a control (see Figure S2A). GFP⁺ and GFP⁻ populations, represented in green and orange, respectively, were interrogated for the expression of each one of the 242 surface markers included in the BD Lyoplate screening panel.

(B and C) Representative example of positive and negative hits, (B) CD77 and (C) CD200, identified based on median fluorescence intensity (MFI) ratio and differential frequency (%) within GFP⁺ and GFP⁻ subpopulations. See Figure S3 for other potential marker candidates.

(D and E) Representative heatmaps depicting the highest-ranked positive or negative hits based on (D) MFI ratio or (E) differential frequency (%) within GFP⁺ and GFP⁻ populations (see Table S1 for heatmap of complete list of 242 markers).