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. 2018 Aug 16;11(3):828–841. doi: 10.1016/j.stemcr.2018.07.007

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© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Validation of hESC-Derived Ventricular Cardiomyocyte Cell-Surface Marker Signature

Multi-color flow cytometry was used to simultaneously analyze the expression of surface markers CD77 and CD200, the intracellular cardiomyocyte marker, cardiac troponin I (cTNI), and GFP at day 25 of cardiac differentiation. Data are representative of a minimum of three independent biological replicates.

(A) MYL2-GFP-positive (green) and -negative (orange) populations were defined within single cells, after exclusion of doublets and debris (left panel). The simultaneous expression of CD200 and CD77 was analyzed within the GFP⁻ population (middle panel, orange) and the GFP⁺ population (right panel, green). See Figure S4 for co-expression of CD77 or CD200 with other marker candidates.

(B) CD77 and CD200 expression was first analyzed within the single-cell population (left panel). The expression of cTNI and MYL2-GFP was then analyzed within the CD77⁻/CD200⁺ population (middle panel, orange) and CD77⁺/CD200⁻ population (right panel, green).