Figure 5.

CD77 Expression Highly Correlates with hESC-Derived Ventricular Cardiomyocyte Phenotype

All analysis is of the same population. Data are representative of a minimum of three independent biological replicates.

(A) Correlation of cTNI and (i) CD77, (ii) SIRPA, or (iii) MYL2-GFP in hESC-derived populations at day 25 of cardiac differentiation by flow cytometry.

(B–E) Representative plots depicting cTNI/MYL2-GFP cell populations (right panel, green plots) at day 25 of cardiac differentiation, after gating on different cell populations defined by distinct surface marker signatures: (B) SIRPA⁺/CD90⁻; (C) SIRPA⁺/CD140b⁻; (D) SIRPA⁺/CD200⁻; and (E) CD77⁺/CD200⁻ (left panel, boxed area).

(F) Left panel: SIRPA⁺/CD77⁻ (yellow) and SIRPA⁺/CD77⁺ (green) cell populations were defined within single cells. Right panel: histograms depicting MYL2-GFP expression within SIRPA⁺/CD77⁻ and SIRPA⁺/CD77⁺ cell populations.