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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: Ultrasound Med Biol. 2018 Jun 23;44(9):1996–2008. doi: 10.1016/j.ultrasmedbio.2018.05.010

Fig. 7.

Fig. 7.

Transmission electron microscopy appearance of E. coli cells from the 5-mL, 5-min exposure treatment combination. At the low magnification (a) (20,000 ×, scale bar is 2 μm), nearly all cells are seen as damaged and missing cytoplasm to various extents; many cell wall fragments are evident. In another field of view (b) (25,000 ×, scale bar is 2 μm), three apparently intact cells share the optical field with three damaged cells and many cellular fragments. The high magnification view (c) (40,000 ×, scale bar is 1 μm) of a smaller portion of the optical field in (b) shows an electron-dense cell essentially indistinguishable from those in unexposed cell images (Fig. 6), a cell in which the cell wall in this plane of section appears intact but the cell is almost free of cytoplasm, and a cell in which an approximately 120-nm hole exists. No pili remain on the bacterial surfaces. The surviving fraction associated with this treatment combination was 0.26 ± 0.07.