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. Author manuscript; available in PMC: 2018 Sep 12.
Published in final edited form as: Immunohorizons. 2018 Aug;2(7):208–215. doi: 10.4049/immunohorizons.1800032

FIGURE 4. Titrated doses of ionomycin does not accurately induce a digital NFAT1 response in OT-I cells.

FIGURE 4.

(A) NFAT1 localization in OT-I nuclei after different doses of ionomycin over the course of 240 min. Representative histograms displaying NFAT1-positive nuclei with indicated dose of ionomycin present in the coculture media. Nuclei were identified as CellTrace Violethi β-tubulinlo (B) For comparison, results of NFAT1-positive OT-I nuclei during coculture with bulk splenocytes pulsed with indicated dose of OVA peptide.