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. 2018 Sep 12;4(9):eaau4640. doi: 10.1126/sciadv.aau4640

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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 3 — (A) Application of whole M. gulosa venom to DRG cells produced a rapid, non–cell-specific increase in [Ca²⁺]_i followed by release of fluorescent dye into the medium. Each trace represents a single cell in the field of view. Snapshots shown of the recording are at 0 s (baseline), 33 s (3 s after addition of venom/fraction), and 150 s. (B) Three different peptides [fractions 8, 24, and 33 (f8, f24, and f33, respectively)] were purified from the whole venom using a single RP-HPLC step. Inset: Reanalysis of purified fractions by RP-HPLC. (C) Fraction 8 (MIITX₂-Mg1a) had no effect on [Ca²⁺]_i in sensory neurons, fraction 24 (MIITX₁-Mg1a) caused an increase in [Ca²⁺]_i in all sensory neurons, while fraction 33 (MIITX₁-Mg2a) induced nonspecific permeabilization of cell membranes.