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. 2018 Sep 12;4(9):eaat7828. doi: 10.1126/sciadv.aat7828

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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 4 — (A) B16F10 (H2B-RFP) cells (5 × 10⁴ cells) intradermally injected into a syngeneic GFP-expressing recipient mouse. Blood collected at time of tumor resection and analyzed by flow cytometry for GFP and RFP expression. RFP⁺GFP⁺ cells were detectible in presorted cell preparations by immunofluorescence. Scale bar, 50 μm. We analyzed GFP-expressing blood by flow cytometry as a negative control for (A) inset. (B) Percentages of fusion hybrids (RFP⁺/GFP⁺) and unfused CTCs (RFP⁺/GFP⁻) expressing the leukocyte antigen CD45 (*P < 0.000002).