Figure 2.

TRIM37 depletion inhibits amino acid-stimulated MTORC1 signaling. (a) HepG2, HEK 293T control, and TRIM37 KD cells were cultured in normal growth media and collected for protein detection via western blotting with the indicated antibodies. T-RPS6KB1 and T-EIF4EBP1 indicate total RPS6KB1 and EIF4EBP1, respectively. ACTB was used as a loading control. (b) HepG2 control and TRIM37 KD cells were incubated in media depleted of amino acids (AA) [38] for 2 h and stimulated with amino acid-containing media for the indicated times, before proteins were detected by western blots. GAPDH was used as a loading control.