Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (A. thaliana) | se-1 mutant | Clarke et al. (1999) | NASC_ID:N3257 | |
| Strain, strain background (A. thaliana) | se-3 mutant | Grigg et al. (2005) | SALK_083196 | |
| Strain, strain background (A. thaliana) | cbp20-1 mutant | Papp et al. (2004) | ||
| Strain, strain background (A. thaliana) | SEPro:SE-3HA | This study | ||
| Antibody | αSE | This study | Raised against the peptide: QDLDAPEE EVTVIDYRSL, 10 µl/ChIP; 1:300 (IP); 1:2000 (WB) | |
| Antibody | αCDT | Abcam | ab817 | 10 µl/ChIP; 1:250 (IP); 1:1000 (WB) |
| Antibody | αCTDI-S2P | Abcam | ab5095 | 10 µl/ChIP; 1:250 (IP); 1:1000 (WB) |
| Antibody | αCTDI-S2P | Abcam | ab5131 | 1:200 (IF) |
| Antibody | αCTDI-S5P | Abcam | ab5408 | 10 µl/ChIP; 1:250 (IP); 1:2000 (WB); 1:200 (IF) |
| Antibody | rat monoclonal IgG1 3F10 anti-HA | Sigma | 000000011867423001 | 1:25 (IF) |
| Antibody | goat anti-rat IgG coupled to Cy3 | Dianova | 112-165-167 | 1:400 (IF) |
| Antibody | goat anti-rabbit antibody coupled to Alexa488 | Dianova | 111-546-047 | 1:400 (IF) |
| Commercial assay or kit | MinElute Reaction Cleanup Kit | Qiagen | 28204 | |
| Commercial assay or kit | QuantiNova SYBR Green PCR Kit | Qiagen | 208052 | |
| Commercial assay or kit | SYBR-Green Maxima | Thermo Fisher Scientific | K0251 | |
| Commercial assay or kit | RevertAid First Strand cDNA Synthesis Kit | Thermo Fisher Scientific | K1621 | |
| Commercial assay or kit | RNeasy Plant Mini Kit | Qiagen | 74904 | |
| Commercial assay or kit | TruSeq ChIP Library Preparation Kit | Illumina | IP-202–1012 | |
| Commercial assay or kit | NEBnext poly(A) mRNA Magnetic Isolation Module | New England Biolab | #7490 | |
| Commercial assay or kit | ScriptSeq v2 RNA-Seq Library Preparation Kit | Illumina, | SSV21124 | |
| Chemical compound, drug | mouse IgG | Santa Cruz | sc-2025 | |
| Chemical compound, drug | rabbit IgG | Santa Cruz, | sc-2027 | |
| Chemical compound, drug | Agarose A/G-Plus beads | Santa Cruz | sc-2003 | |
| Chemical compound, drug | protease inhibitor cocktail | SIGMA | P9599 | |
| Chemical compound, drug | Phosphatase Inhibitor Cocktail 2 | SIGMA | P5726 | |
| Chemical compound, drug | Phosphatase Inhibitor Cocktail 23 | SIGMA | P0044 | |
| Chemical compound, drug | protein A-Agarose | Roche | 11134515001 | |
| Chemical compound, drug | protein G-Agarose | Roche | 11243233001 | |
| Chemical compound, drug | cOmplete, EDTA-free | Sigma | 000000011873580001 |