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Dot/Icm effectors control the localization of LC3B to the CCV. (a) HeLa cells were infected with the indicated Coxiella strains (red) for 3 days, then stained for LC3B (green), and nuclei (DAPI, blue). The CCVs are highlighted with white asterisks. Scale bars: 10 µm. (b) Quantification of the percentage of HeLa cells that have LC3B-positive CCVs. At least 100 infected cells for each of 3 independent experiments were scored for recruitment of LC3B. Error bars represent SD.
