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. 2018 Jul 28;14(10):1726–1741. doi: 10.1080/15548627.2018.1483807

Figure 4.

Figure 4.

Expression levels of bone formation and resorption markers in osteoblast-specific Atg7 knockout mice during adult and weanling stages. RNA was extracted from ribs of P21 mice for weanling stages and calvaria of 22-wk for adult stage. (a) RT-qPCR for bone formation markers (Runx2, Spp1, Bglap, Alpl, and Sp7) in mice during adult stage. (b) mRNAs coding for Tnfrsf11b, Tnfsf11, Tgfb1, and Bmp4 expressed by osteoblasts during adult stage. (c) RT-qPCR for bone resorption markers expressed by osteoclasts (Mmp9, Ctsk, and Acp5) during adult stage. (d and e) Serum TNFSF11 and TNFRSF11B levels during adult stage. (f) RT-qPCR for Runx2, Spp1, Ctsk, Acp5, Tnfrsf11b and Tnfsf11 in P21 mice. (g) RUNX2 protein in tibia from weanling mice assessed by immunohistochemistry. Scale bars: 100 µm. *Statistically significant difference between control and cKO (n = 4 to 6/group), and results were presented as gene expression levels in all groups normalized to controls (mean ± SD, Student t test; *P < 0.05).