Figure 5.

XFp analysis of glycolysis stress shows acute elimination of glycolysis-associated acidification with acute MB exposure in neuroblastoma cells. Seahorse XFp analysis using the Glycolysis Stress Test kit was performed with acute injection of DMSO or MB at varying concentrations. A, The time course and live measurement of ECAR with injections of stressor compounds and substrates (n = 7–9, mean ± SEM). Individual parameters of glycolysis (B) nonglycolytic acidification, (C) glycolysis, (D) glycolytic capacity, and (E) glycolytic reserve were calculated according to manufacturer’s protocol.