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50 µM MB reduces cellular ATP and NAD in neuroblastoma cells. A, CellTiter-Glo chemiluminescence assay was performed on neuroblastoma cells at 50 µM MB or 0.5% DMSO for 1 h of treatment and displayed as percent control (n = 10, mean ± SEM). B, LC/MS/MS nucleotide assay was performed with DMSO and 50 µM MB (n = 6, mean ± SEM). Percent control is reported for 50 µM MB for (B) Cellular ATP and (C) Cellular NAD.
