Table 1.

Protoplast yield (per root tip) and average size obtained using different enzyme cocktails and pretreatment combinations. The number of small protoplast was determined to assess the protocol efficiency in digesting inner meristematic tissue.

	No. of protplasts per root tip	Average protoplast diameter (μm)	% of small protoplast (<15 μm)	Absolute no. of small protoplasts (<15 μm)
L-cysteine + Enzyme cocktail 1 (Seen et al.)	4,524	24	10	456
Enzyme cocktail 2 (Evrard et al.)	3,151	18	30.3	956
L-cysteine + Enzyme cocktail 2 (protocol reported in this unit)	23,500	21	23.1	5,450