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Ear meristem protoplast yield (per mg of tissue) and size obtained with enzyme cocktail 2.
| No. of protplasts per mg ear meristem tissue | Average protoplast diameter (μm) | % of small protoplast (<15 μm) | Absolute no. of small protoplasts (<15 μm) | |
|---|---|---|---|---|
| 15min | 1,967 | 14.8 | 55% | 1,082 |
| 0.5hr | 5,467 | 15.0 | 60% | 3,280 |
| 1hr | 7,133 | 16.6 | 58% | 4,137 |
| 2hr | 11,800 | 18.3 | 27% | 3,186 |
| 3hr | 10,800 | 16.9 | 36% | 3,888 |
| 4hr | 9,533 | 17.4 | 40% | 3,813 |
